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Category:Arbeitsbücher
Category:Children's non-fiction books
Category:Children's books by Hans ZimmerGenetic analysis of rat Cdc45a: implications for meiotic recombination.
Gene expression is thought to be controlled by epigenetic, as well as genetic, mechanisms. In this study, a genetic analysis of the gene Cdc45a in the rat was conducted, and the results are discussed in relation to the control of gene expression and to the regulation of meiotic recombination. We report here the isolation and characterization of a cosmid containing the rat Cdc45a gene and flanking DNA sequences. The gene is located on rat chromosome 11 and encodes a putative chromosomal co-factor of the DNA replication and repair factor PCNA. We were able to detect a transcript of Cdc45a in rat tissues and show that the gene is expressed throughout the male meiotic cycle and is thus also transcribed during oogenesis. We also describe the first generation of an animal mutant line in which a small deletion in the rat Cdc45a gene is responsible for the recessive lethal Wisc strain mutant phenotype (Tajima et al., 1998: Nature 392:205-208). The mutant phenotype is characterised by complete male sterility and by the lack of the majority of oocytes at the preantral stage in females. The phenotype appears to be due to a defect in meiotic recombination.To gain an understanding of the glycolytic metabolism of the normal and malignant cells in the human colon. Study of the metabolic characteristics of normal and malignant colonic tissue in vitro and ex vivo. Quantitative analysis of the glycolytic activity of normal and malignant tissue and cell lines, comparing the extent of aerobic and anaerobic glycolysis. Assessment of the metabolic activities of oxygen-dependent and -independent enzymes, ac619d1d87
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